University of Toronto
UTSC Harmful Algae Analytical Unit
The UTSC Harmful Algae Analytical Unit generates baseline and time-sensitive data to understand the drivers influencing phytoplankton dynamics (community composition, ecology, and physiology), with an emphasis on examining harmful algal blooms. In addition, it provides open-access data obtained from a combination of field and laboratory-based techniques to investigate aquatic microbial communities and contains modern analytical equipment for algal culturing, algal class analyses (e.g., PhycoLabAnalyser, fluoroprobe and PHYTO-PAM), phytoplankton identification and enumeration (e.g., high-resolution microscopy, FlowCam), and toxin analyses.
CytoFLEX Flow Cytometer (Beckman Coulter)
UTHAAL-ML-1
Cell analysis system used for the qualitative and quantitative measurement of biological and physical properties of cells and other particles. Application flexibility, including an optional 96-well Plate Loader, 13 bandpass filters, and two lasers (Blue and Red).
PhytoCyt Flow Cytometer (Turner Designs) Portable
UTHAAL-ML-2 | UTHAAL-ML-3
Cell analysis system used for many applications (e.g., cell counting, viability assays, physiological characterization, etc.). The optical filters and lasers have been optimized to detect the endogenous fluorophores common to phytoplankton, including phycoerythrin, chlorophyll and phycocyanins. One of the detectors has been optimized for green fluorescence detection, which allows labelling with exogenous reagents.
IX73 Inverted LED Fluorescence Microscope (Olympus)
UTHAAL-ML-4
Fluorescence microscopy is a major tool used to monitor cell physiology and aid in cell identification. The system has imaging capabilities for standard imaging tasks.
BX 42Fl Light Microscopy (Olympus)
UTHAAL-ML-5
Suitable for various applications and has imaging capabilities for standard imaging tasks.
ECLIPSE Ts2 Inverted Microscopes (Nikon)
UTHAAL-ML-6
Suitable for various applications and has imaging capabilities for standard imaging tasks.
Multiskan™ FC Microplate Photometer (Thermo Scientific) (340–850 nm)
UTHAAL-ML-7
Provides fast and accurate measurements for 96-well plates. The wavelength range of 340– 850 nm and is used for a wide variety of applications such as ELISA immunoassays, protein quantification, endotoxin, cytotoxicity and proliferation assays, enzyme assays and growth curves.
Cary 300 UV-Visible Spectrophotometer
UTHAAL-ML-8
The wavelength range of 190–900 nm and is used for a wide variety of applications (wavelength accuracy ± 0.2 nm).
Cary Eclipse is a Fluorescence Spectrophotometer
UTHAAL-ML-9
Flexible fluorescence spectrometer with multiple data collection modes, including fluorescence, phosphorescence, chemiluminescence, bioluminescence, and time-resolved phosphorescence. Operational emission: 200 - 900 nm Operational excitation: 200 - 900 nm
Innova® S44i - Stackable Incubator Shaker (Three units available)
UTHAAL-ML-10 | UTHAAL-ML-11
Temperature-controlled (refrigerated) shaker allowing the use of high-volume flasks. Photosynthetic LED light bank provides the ability to evenly light the entire platform across a wide intensity range.
Panasonic Growth Incubators (Two units available)
UTHAAL-ML-12 | UTHAAL-ML-13
Temperature-controlled (refrigerated) unit allowing the use of high-volume flasks. Ability to control light intensity and light cycles.
Milli-Q® Benchtop Lab Water Purification Systems
UTHAAL-ML-14
Water purification system providing ultrapure water for laboratory needs (e.g., buffers and bacterial media preparation).
Multi-Cultivator MC 1000-OD
UTHAAL-ML-15 | UTHAAL-ML-16
Small-scale cultivation device developed for the cultivation of multiple samples. The instrument is primarily intended for the synchronous growth of algae, bacteria or cyanobacteria under defined conditions with a wide range of applications (i.e. toxicological and eco-toxicological testing, optimization of cultivation conditions, phenotypization of various strains).
Purifier Logic+ Class II, Type A2 Biosafety Cabinets
UTHAAL-ML-17
Workstation provides personnel, product, and environmental protection from hazardous particulates such as agents that require Biosafety Level 1 or 2.
